Jpn. J. Infect. Dis., 65 (5), 430-432, 2012

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Short Communication

Detection of Five Rash-Associated Viruses Using Multiplex Real-Time PCR during 2006–2011

Atsushi Kaida*, Hideyuki Kubo, Jun-ichiro Sekiguchi, Minori Ohyama, Kaoru Goto, Atsushi Hase, and Nobuhiro Iritani

Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, Osaka 543-0026, Japan

(Received March 12, 2012. Accepted June 11, 2012)

*Corresponding author: Mailing address: Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, 8-34 Tojo-cho, Tennoji-ku, Osaka 543-0026, Japan. Tel: +81-6-6771-3147, Fax: +81-6-6772-0676, E-mail: このメールアドレスはスパムボットから保護されています。閲覧するにはJavaScriptを有効にする必要があります。

SUMMARY: Many viruses have been reported to be associated with rash development. Multiplex real-time PCR was used to investigate the presence of 5 viruses associated with rashes: measles virus (MV), rubella virus (RV), human parvovirus B19 (PVB19), human herpes virus 6 (HHV-6), and HHV-7. A total of 187 clinical specimens from 169 patients with erythema were collected between January 2006 and December 2011. Virus-positive specimens were as follows: MV (n = 23), PVB19 (n = 8), RV (n = 2), HHV-6 (n = 5), HHV-7 (n = 1), MV and PVB19 (n = 1), and HHV-6 and HHV-7 (n = 1). All of the MV-positive specimens were collected in 2007 and the strains whose sequence were available (21/24, 87.5%) were of genotype D5. The results indicate that multiplex real-time PCR might be a useful screening method for detecting and differentiating rash-associated viruses in clinical specimens.