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  <TITLE>Chiba et al., Construction of a Pair of Practical Nocardia-Escherichia coli Shuttle Vectors</TITLE>

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<P>Jpn. J. Infect. Dis., 60 (1), 45-47, 2007</P>


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<P>Original Article</P>


<P><B><FONT SIZE="+2">Construction of a Pair of Practical <I>Nocardia-Escherichia coli</I> Shuttle Vectors</FONT></B></P>


<P><B>Kazuhiro Chiba, Yasutaka Hoshino, Keiko Ishino, Takahisa Kogure<FONT SIZE="-1"><sup>1</sup></Font>, 

Yuzuru Mikami<FONT SIZE="-1"><sup>1</sup></Font>, Yoshimasa Uehara and Jun Ishikawa*</B></P>


<P>Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162-8640, and

<FONT SIZE="-1"><sup>1</sup></Font>Department of Molecular Function, Research Center for Pathogenic Fungi and

Microbial Toxicoses, Chiba University, Chiba 260-8673, Japan</P>


<P>(Received October 3, 2006. Accepted November 28, 2006)</P>


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<P>*Corresponding author: Mailing address: Department of Bioactive Molecules, National Institute of

Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo 162-8640, Japan. Tel: +81-3-5285-1111, 

Fax: +81-3-5285-1272, E-mail: jun@nih.go.jp</P>


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<P><B>SUMMARY</B>: We constructed a pair of <I>Nocardia-Escherichia coli</I> shuttle vectors, 

pNV18 and pNV19, by combining the mycobacterial plasmid pAL5000 with the <I>E. coli</I> vector pK18 or pK19. 

These vectors have a number of useful features, including small size (4.4 kb), a multiple cloning site, 

and blue/white selection. To our knowledge, pNV18 and pNV19 are the first cloning vectors for practical use in <I>Nocardia</I> spp.</P>


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