MAP kinase protocol

[Alex Lyakhovitch <loally@uta.fi>]

Hi,

Is there anybody who did MAP kinase assay with P-32-ATP, MBP, filter binding? 
What buffer should I use for best results?

I used 50mM Hepes-HCl pH8.0, 2.0 mMDTT, 0.1 EGTA, 5mM Mg(Ch3COO) buffer but
it seems to me that the binding system does not work.

Can anybode give me a good hint why it could happen?

Thanks in advance,
Regards,
Alex
loally@uta.fi

Alex Lyakhovich, M.D.
University of Tampere
Medical School
Box 607 
FIN-33101 Tampere
Finland
--------------------
tel. 358-3-2156640
fax 358-31-156170